Physicochemical Alterations in Liposomes Induced by Low Concentrations of Synthetic Antioxidant Potassium Phenosan

摘要

We have recently shown by spin probe ESR that synthetic antioxidant potassium p-(4-hydroxy-3,5-di-tert-butylphenyl)propionate (potassium phenosan, PP) can modify the structural state of surface (8 A) and deep-lying (20-22 A) regions of lipids of plasmatic membranes isolated from mouse liver cells in a wide concentration range (10~(-5)-10~(-21) M) in vitro [1, 2]. Nonlinear bimodal dose-effect dependences were obtained for parameters that characterize the micro-viscosity of lipid bilayer (τ_c) in the location region of nitroxyl fragment of 16-doxylstearic acid (C_(16)) probe and for ordering parameter (S) that characterizes the rigidity of surface lipid layers in the location area of 5-doxylstearic acid (C5) probe.