Analysis of Expression of Genes Specific for Pluripotent and Primordial Germ Cells in Human and Mouse Embryonic Stem Cell Lines

摘要

Permanent lines of embryonic stem cells (ESCs) of humans and animals are promising cell models for fundamental, biomedical, pharmacological, and lexicological studies, Today, the world collection of human ESC lines numbers approximately 300 lines, including the reconstructed lines obtained using the technology of somatic cell nucleus transfer into the enucleated oocyte and the lines carrying genetic lesions [1-4]. Human ESC lines obtained at Russian institutes (Institute of Cytology and Institute ofGene Biology, Russian Academy of Sciences) were characterized earlier using the standard tests detecting the expression of the embryonic surface antigens SSEA3 and SSEA4, the transcription factors OCT4 and NANOG, as well as the activity of alkaline phosphatase and telomerase [4].