Interaction of cyanine dyes with nucleic acids.XX.New methods for the preparation of fluorescent probes based on benzothiazol-4-[2,6-dimethylpyridinium]cyanine dyes

摘要

Two new methods of preparing fluorescent dyes containiny affinity-modifying groups for nucleic acid detection are proposed.One method of preparation is based on thereaction of the pyrylium monomethine cyanine dye 2-(2,6-dimethyl-pyran-4-ilidenmethyl)-3-methyl-benzothiazolium perchlorate with primary amines.The second method ofpreparation involves the amidation of carboxy substituted dyes with appropriate amines.