Synthesis of novel fluorescent styryl dyes based on the imidazo[1,2-a]pyridinium chromophore and their spectral-fluorescent properties in the presence of nucleic acids and proteins

摘要

The imidazo[1,2-a]pyridinium heterocyclic system was used to prepare styryl dyes.Improved synthetic methods were proposed for the parent imidazo[1,2-a]pyridines and their corresponding quaternary salts.The standard method for preparing styrylcyanines was modified for the synthesis of the target imidazo[1,2-a]pyridinium dyes.Spectral-luminescent properties of the obtained dyes in free state and in the presence of nucleic acids,BSA,and BSA/detergent system were studied.7-[2-(4-Dihexylaminophenyl)-1-ethenyl]-2-(2,4-dimethoxyphenyl)-1-ethylimidazo[1,2-a]pyridin-1-ium iodide(SIP-8)containing C_6 aliphatic tails and 2,4-methoxy substituents in the 2-phenyl ring exhibited specificity to BSA.