Effect of tocotrienols on iron-induced renal dysfunction and oxidative stress in rats

摘要

Ferric nitrilotriacetate (Fe-NTA) is a well-established nephrotoxic agent. This study was designed to investigate the modulatory effect of the subacute administration of tocotrienol-rich fraction (T3),a product from palm oil,and a-tocopherol (T) on Fe-NTA-induced renal injury and oxidative stress. Fe-NTA administration markedly increased blood urea nitrogen (BUN) and serum creatinine level,which was coupled with a marked lipid peroxidation,reduced activity of glutathione levels,and morphological alterations in rat kidney. Pretreatment with T3 (50 mg/kg/day) and T (50 mg/kg/day) for 7 days before Fe-NTA administration significantly reduced the serum creatinine and BUN levels,reduced lipid peroxidation in a significant manner,and restored levels of reduced glutathione and superoxide dismutase.T3 pretreatment also attenuated the serum tumor necrosis factor-alpha levels,as compared to pretreatment with T,and restored normal renal morphology. These findings suggest a strong correlation between iron-induced oxidative stress and renal dysfunction and point toward the protective effects of T3 in Fe-NTA-induced renal injury.