Genomic cloning of a heat shock cognate 71-1 gene (HSC71-1) from the hermaphroditic fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)

摘要

The self-fertilizing fish Rivulus marmoratus ( R. marmoratus ) heat shock cognate 71 ( HSC71 ) gene was cloned and characterized recently ( Park et al., 2001 ). Here, we report the isolation of a homologue of the R. marmoratus HSC71 gene via screening of an R. marmoratus genomic DNA library. A 12,591 bp genomic fragment was sequenced and found to contain a 2844 bp open reading frame that consisted of 8 exons and showed high similarity to the previously reported R. marmoratus HSC71 gene. The two genes differed slightly at exons 5 and 8, and intron 3. On a deduced amino acid sequence level, the two R. marmoratus HSC71 genes were highly similar (89.3% in amino acid residues). In this paper, the author presented a homologous gene ( R. marmoratus HSC71-1 ) similar to R. marmoratus HSC71 gene.