首页> 外文期刊>Chromosome research: An international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology >Avian comparative genomics: reciprocal chromosome painting between domestic chicken (Gallus gallus) and the stone curlew (Burhinus oedicnemus, Charadriiformes)--an atypical species with low diploid number.
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Avian comparative genomics: reciprocal chromosome painting between domestic chicken (Gallus gallus) and the stone curlew (Burhinus oedicnemus, Charadriiformes)--an atypical species with low diploid number.

机译:禽类比较基因组学:家禽(鸡)和curl(Burhinus oedicnemus,Charadriiformes)之间的染色体互画(一种低二倍体数的非典型物种)。

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摘要

The chicken is the most extensively studied species in birds and thus constitutes an ideal reference for comparative genomics in birds. Comparative cytogenetic studies indicate that the chicken has retained many chromosome characters of the ancestral avian karyotype. The homology between chicken macrochromosomes (1-9 and Z) and their counterparts in more than 40 avian species of 10 different orders has been established by chromosome painting. However, the avian homologues of chicken microchromosomes remain to be defined. Moreover, no reciprocal chromosome painting in birds has been performed due to the lack of chromosome-specific probes from other avian species. Here we have generated a set of chromosome-specific paints using flow cytometry that cover the whole genome of the stone curlew (Burhinus oedicnemus, Charadriiformes), a species with one of the lowest diploid number so far reported in birds, as well as paints from more microchromosomes of the chicken. A genome-wide comparative map between the chicken and the stone curlew has been constructed for the first time based on reciprocal chromosome painting. The results indicate that extensive chromosome fusions underlie the sharp decrease in the diploid number in the stone curlew. To a lesser extent, chromosome fissions and inversions occurred also during the evolution of the stone curlew. It is anticipated that this complete set of chromosome painting probes from the first Neoaves species will become an invaluable tool for avian comparative cytogenetics.
机译:鸡是鸟类中研究最广泛的物种,因此可作为鸟类比较基因组学的理想参考。比较的细胞遗传学研究表明,这只鸡保留了许多祖先鸟核型的染色体特征。通过染色体涂染已经建立了鸡大染色体(1-9和Z)与10种不同顺序的40多种鸟类中对应物之间的同源性。但是,鸡微染色体的禽同源物仍有待确定。此外,由于缺乏其他禽类的染色体特异性探针,因此未在鸟类中进行互补的染色体绘画。在这里,我们使用流式细胞仪生成了一组特定于染色体的油漆,这些油漆覆盖了石curl的整个基因组(Burhinus oedicnemus,Charadriiformes),这是迄今为止鸟类中二倍体数最低的物种,以及鸡肉的更多微染色体。鸡和石curl之间的全基因组比较图谱是根据对立的染色体绘画首次构建的。结果表明,广泛的染色体融合是石curl中二倍体数量急剧下降的基础。在较小的程度上,在石curl的进化过程中也发生了染色体裂变和倒置。可以预料,来自第一个Neoaves物种的这套完整的染色体绘画探针将成为禽类比较细胞遗传学的宝贵工具。

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