Establishment of Embryonic Stem Cell Lines Derived from Outbred Mouse Embryos and Production of Chimeras

摘要

The aim of the present investigation was to determine if embryonic stem (ES) cells could he isolated from outbred mouse embryos (KM) and if chimeras could be produccdly using outbred ES cells. Three ES cell lines, designated KE1, KE2, and KE5, were isolated from 5 Kunming albino blastocysts. Normal diploid composition of these cell lines was above 70%. By using C57BL/6J and 615 blastocysts as host embryos, one chimera was obtained in living pups. It was shown for the first time that chimeras can beproduced by using outbred ES cells. This work implies that to establish ES cell lines from other animal embryos is possible. More interestingly, white color dots from ES cells on the coat of this chimcric mouse enlarged a lot after half a year, indicatedthat ES cells were inhibited by cells from outbred mouse or the cells of outbred mouse grew vigiously.