My interest in protein breakdown as a research problem began in 1955 at about the time I joined the Biochemistry Department of Yale University.It was known that proteins break down intracellularly in the mature animal.In 1955 I learned from Melvin Simpson about experiments he had published two years earlier showing that a variety of conditions that should lower the ATP level of liver slices(anaerobiosis,cyanide,2,4-dinitrophenol)decreased the rate of liberation of labeled methionine from protein of rat liver slices.
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