Comparative susceptibilities of six insect cell lines to infectionby Malacosoma neustria nucleopolyhedrovirus (ManeNPV)

摘要

In this study, 6 lepidopteran cell lines derived from Trichoplusia ni (Tn5), Lymantria dispar (LdElta), Bombyxmori (Bm-5), Spodoptera frugiperda (Sf21), Spodoptera exigua (Se-1), and Choristoneura fumiferana (Cf-124T) wereinvestigated for susceptibility to Malacosoma neustria nucleopolyhedrovirus (ManeNPV) isolated in Turkey. Infection ofMalacosoma disstria (Md203) cell line by ManeNPV was used as a control. In ManeNPV-Md203 infection, polyhedralinclusion bodies (PIBs) were detected at 36 h p.i. and observed in all infected cells by 120 h p.i., and the infectious virustiter increased 10~3-fold. At 24 h p.i., the efficient replication of viral DNA was detected. In this study, we determined thatTn5 and LdElta cells were productive to ManeNPV, and the replication of viral DNA occurred at 24 h p.i. PIB productionswere determined in both cell lines at 36 h p.i., and the infectious virus titer in both cell lines increased 10-fold. By contrast,infections of Sf21 cells were not found productive. However, infected cells had apoptosis-like structures. Finally, nocytopathic effects, inclusion bodies, and viral DNA replication were detected in infected Bm-5, Se-1, and Cf-124T celllines. Therefore, these cell lines were accepted as non-productive. This is the first study on the host specifity of ManeNPVin vitro. Our results suggest that Tn5 and LdElta cell lines can provide an important model for studying ManeNPV in vitrosystem as in the Md203 cell line system.