Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12

摘要

The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmara?. Enzyme synthesis occurred at 20-55 oC with an optimum of 42 oC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.