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Effects of Mercury and Chromium on Peroxidase and IAA Oxidase Enzymes in the Seedlings of Phaseolus vulgaris

机译:汞和铬对菜豆幼苗过氧化物酶和IAA氧化酶的影响

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摘要

Phaseolus seedlings (Phaseolus vulgaris L.) in light conditions were treated with 2 different concentrations of 2 heavy metals, mercury (0.05 mM and 0.4 mM) and chromium (0.5 and 1.0 mM). Mercury was given in the form of HgCl_2 and chromium was given in the form of K_2Cr_2O_7. Peroxidase activity was measured with 4 different hydrogen donors (ferulic acid, caffeic acid, pyrocatechol and pyrogallol). Both mercury and chromium inhibited root and hypocotyl length. The inhibition was about 50%-80%, depending on the concentration of the heavy metal. Peroxidase and IAA oxidase activities showed a clear inverse relation with growth. Seedlings treated with a lower concentration of mercury had similar activities to those treated with higher concentrations of chromium. The role of peroxidase and IAA oxidase activities in defense mechanisms in response to heavy metal toxicity is discussed.
机译:用两种不同浓度的2种重金属,汞(0.05 mM和0.4 mM)和铬(0.5和1.0 mM)处理菜豆在轻的条件下的菜豆(Phaseolus vulgaris L.)。汞以HgCl_2的形式给出,铬以K_2Cr_2O_7的形式给出。用4种不同的氢供体(阿魏酸,咖啡酸,邻苯二酚和邻苯三酚)测量过氧化物酶活性。汞和铬均抑制根和下胚轴长度。抑制作用约为50%-80%,具体取决于重金属的浓度。过氧化物酶和IAA氧化酶活性与生长呈明显的负相关。用较低浓度的汞处理的幼苗具有与较高浓度的铬处理的幼苗相似的活性。讨论了过氧化物酶和IAA氧化酶活性在防御机制对重金属毒性反应中的作用。

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