Effect of different phenolic compounds on alpha-amylase activity: screening by microplate-reader based kinetic assay.

摘要

The inhibitory effect of different polyphenolic plant compounds on alpha-amylase activity was investigated in vitro. A kinetic assay was performed using 96-well-plates. Acarbose was used as positive control (IC50: 23.2 microM). Some of the tested compounds, occurring in plants traditionally used in anti-diabetic tea species, showed an inhibition of the enzyme in physiological concentrations, e.g. luteolin, tannic acid, and isochlorogenic acid.