Evaluation of different plasmid DNA delivery systems for immunization against HER2eu in a transgenic murine model of mammary carcinoma

摘要

Studies of DNA vaccination against HER2eu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors; scarce information, however, has been provided to identify the procedure of DNA administration that more effectively contributes to the activation of immune system against spontaneously arising HER2eu-positive tumors. We compared the effectiveness of different procedures of DNA vaccine delivery (intradermic injection (ID), gene gun (GG) delivery and intramuscular injection (IM) alone or with electroporation) in a murine transgenic model of mammary carcinoma overexpressing HER2eu. We highlighted the role of DNA delivery system in the success of DNA vaccination showing that, among the analysed methods, intramuscular injection of the vaccine, particularly when associated to electroporation, elicits a better protection against HER2eu spontaneous tumor development inducing antibody and cell-mediated immune responsiveness against HER2eu and a Th1 polarization of the immune response.