首页> 外文期刊>Zoonoses and Public Health >Detection of the flor gene in a diversity of florfenicol resistant gram-negative bacilli from freshwater salmon farms in Chile.
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Detection of the flor gene in a diversity of florfenicol resistant gram-negative bacilli from freshwater salmon farms in Chile.

机译:在智利淡水鲑鱼养殖场中检测出多种耐氟苯尼考的​​革兰氏阴性杆菌中的flor基因。

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Florfenicol is an important antibiotic in veterinary medicine that is used extensively in aquaculture, including salmon farming in Chile. We analysed a set of 119 florfenicol-resistant Gram-negative bacilli from seven freshwater Chilean salmon farms for the molecular determinants involved in the florfenicol resistance. Ninety-seven of these strains were glucose non-fermenting bacilli, mainly belonging to the Pseudomonas genus, whereas 22 strains were glucose-fermenters. The floR gene was detected in 26 strains (21.8%) that had been isolated from three of the seven salmon farms. Most of the floR-carrying strains were glucose fermenters (21 strains), and most of the floR-carrying strains were also resistant to streptomycin, chloramphenicol and oxytetracycline. The minimum inhibitory concentrations against florfenicol were assessed in the presence and absence of the efflux pump inhibitor Phe-Arg- beta-naphthylamide (MC-207,110). There was evidence that in the majority of non-fermenting bacteria (82 strains), florfenicol resistance was at least partially mediated by non-specific efflux pump systems. Given the diversity of antibiotic resistance patterns observed in this study in the floR-positive isolates, a single antibiotic has the potential to co-select for a diversity of resistances. For this reason, human health as well as animal health can potentially be impacted by the use of antibiotics in aquaculture. To assess this potential risk, future studies should focus on the ability of different antibiotics used in aquatic environments to co-select for multiple resistances, the molecular basis of this diversity of resistance, and whether the genes conferring resistance can be transferred to other bacteria, including those of human health concern.
机译:氟苯尼考是兽医学中一种重要的抗生素,广泛用于水产养殖,包括智利的鲑鱼养殖。我们分析了一组来自七个淡水智利鲑鱼养殖场的119例对氟苯尼考耐药的革兰氏阴性杆菌,以确定其与氟苯尼考耐药性有关的分子决定因素。这些菌株中的九十七个是葡萄糖非发酵菌,主要属​​于假单胞菌属,而二十二个菌株是葡萄糖发酵菌。从七个鲑鱼养殖场中的三个分离出的26个菌株(21.8%)中检测到了floR基因。大多数带有floR的菌株是葡萄糖发酵罐(21个菌株),而且大多数带有floR的菌株也对链霉素,氯霉素和土霉素有抗性。在有和没有外排泵抑制剂Phe-Arg-β-萘酰胺(MC-207,110)的存在下,评估了对氟苯尼考的​​最低抑制浓度。有证据表明,在大多数非发酵细菌(82个菌株)中,氟苯尼考耐药至少部分是由非特异性外排泵系统介导的。考虑到在本研究中在floR阳性分离物中观察到的抗生素耐药性模式的多样性,单一抗生素有可能共同选择多种耐药性。因此,水产养殖中使用抗生素可能会影响人类健康以及动物健康。为了评估这种潜在风险,未来的研究应集中在水生环境中使用的不同抗生素共同选择多重抗药性的能力,这种抗药性多样性的分子基础,以及赋予抗药性的基因是否可以转移到其他细菌上,包括那些与人类健康有关的问题。

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