Artefacts in H-1 NMR-based metabolomic studies on cell cultures

摘要

Object Metabolomic studies on cultured cells involve assays of cell extracts and culture medium, both of which are often performed by H-1 NMR. Cell culture is nowadays performed in plastic dishes or flasks, and the extraction of metabolites from the cells is typically performed with perchloric acid, methanol-chloroform, or acetonitrile, ideally while the cells are still adherent to the culture dish. We conducted this investigation to identify contaminants from cell culture plasticware in metabolomic studies.