A routine method of DNA-extraction from extremely small metazoans, e.g.single rotifer specimens for RAPD-PCR analyses

摘要

A simple and efficient method has been developed for isolating genomic DNA from single specimens of extremely small metazoan animals, e.g.rotifers (Rotifera: Monogononta). The amount and quaity of the extracted DNA allows the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to be employed with up to three random primers per specimen. Reprducibility of RAPD-fingerprints has been checked and verified. Exemplarily, RAPD patterns of three Brachionus species (B.angularis, B.calyciflours, B.urceolaris) amplified with the primer OPA 07 are demonstrated.