A novel and efficient nicotine vaccine using nano-lipoplex as a delivery vehicle

摘要

Abbreviations: APC, antigen presenting cells; BSA, bovine serum albumin; DC, dendritic cell; DLS, dynamic light scattering;DOTAP, l,2-dioleoyl-3-trimethylammonium-propane (chloride salt); DSPE-PEG(2000)-maleimide, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(maleimide[polyethylene glycol]-2000) (ammonium salt); ELISA, enzyme-linked immunosorbent assay; FITC, fluorescein isothiocyanate-dextran; rEPA, recombinant exotoxin A; NBL, Nic-BSA-Liposome; Nic, rac-trans 3'-hydroxymethylnicotine hemisuccinate; NicAb, anti-Nic antibodies; Nic-BSA, nicotine-BSA conjugate; RES, reticulo-endothelial system; Rhod B, Rhodamine B isothiocyanate; TEM, transmission electron microscope; TT, tetanus toxoid