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The Production of Callus, Shoot, and Rooted Plantlets of Rhododendron catawbiense 'English Roseum' from Florets

机译:小花杜鹃杜鹃“英国玫瑰”的愈伤组织,芽和生根苗的生产

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摘要

The Rhododendron catawbiense (Michx.) varieties 'Roseum Elegans' and 'English Roseum' are the primary varieties of rhododendron sold in the northeastern United States; however, very little research has been published on their ability to produce shoots, roots, or plantlets in tissue culture. The suitability of two rhododendron varieties (including 'English Roseum') was examined using two different combinations of cytokinin and auxin in Anderson's rhododendron medium. It was determined that the variety'Scintillation' performed poorly regardless of the media conditions used; however, 'English Roseum' produced callus and shoots using Anderson's medium and 4.0 mg L~(-1) IAA and 15 mg-L~(-1) 2iP. Rooted plantlets were produced using a rooting medium containing Anderson's medium and 4 mg-L~(-1) NAA. The ability of 'English Roseum' to produce 35% successful plantlets under these conditions suggests that this variety is moderately suitable for micropropagation purposes.
机译:杜鹃花杜鹃(Michx。)品种“ Roseum Elegans”和“ English Roseum”是在美国东北部销售的杜鹃花的主要品种。然而,关于它们在组织培养中产生芽,根或小植株的能力的研究很少。使用细胞分裂素和生长素的两种不同组合,在安德森杜鹃花培养基中检测了两个杜鹃花品种(包括“英国玫瑰”)的适用性。可以确定,无论使用哪种培养基,该品种的“闪烁”效果都不好。然而,“英国玫瑰”使用安德森氏培养基和4.0 mg L〜(-1)IAA和15 mg-L〜(-1)2iP产生愈伤组织和芽。使用含有安德森氏培养基和4 mg-L〜(-1)NAA的生根培养基可以生根。在这些条件下,“英国蔷薇”能够成功繁殖出35%的苗,这表明该品种适度适合于微繁殖。

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