Vitrification of Gladiolus Shoot Tips from Cormels

摘要

Gladiolus shoot tips, 1-2 mm, were excised from in vitro and greenhouse-grown cormels of cultivar 'Peter Pears,' in vitro-grown cormels of 'Jenny Lee,' field-grown cormels of the breeding lines 02-943A, 02-900, 02-926, and field-grown cormels of the cultivar 'Double Delight'. The major factors that affected growth of shoot tips following vitrification were size and dormancy of the excised shoot tip, cultivar, and time in Plant Vitrification Solution 2 (PVS2). The highest survivals of shoot tips was achieved using either greenhouse-grown 'Peter Pears' (77%) and field-grown 02-943A (55%). Recovery of viable shoot tips was best with incubation times of 30 and 60 minutes in PVS2 for 'Jenny Lee' and 02-943A, respectively. All shoots that have grown following vitrification were phenotypically normal in the greenhouse.