Effects of auxin and sucrose on cell growth and production of phenolic compounds in cell suspension culture of Panax ginseng C.A. Meyer.

摘要

The effect MS medium supplemented with auxins (2,4-D, IBA or IAA) and sucrose (3, 5 or 7%) on the production of phenolic compounds by cell suspensions of P. ginseng [P. pseudoginseng] was investigated. Cell growth was best with 2,4-D (4.38 g/flask)and worst with IAA (1.89 g/flask). PAL activity and phenolic content/flask were lowest with IBA. PAL activity was highest with IAA. As IAA concentrations increased from 10-6 to 10-4 M, cell growth increased, phenolic compound content/flask increased (from 2.06 to 5.01 mug/flask), but PAL activity decreased. Cell growth was best on a medium containing 3% sucrose, but the highest production of phenolic compounds was observed after 3 weeks on a medium containing 5% sucrose.