Micropropagation from Stem Segments of Salt Tolerant Jojoba Seedlings

摘要

In order to produce shoots from nodal segments ot salt tolerant jojoba seedlings, the established explants on MS basal medium containing benzyladenine (BA) and naphthaleneacetic acid (NAA), were transferred to 1/2 MS medium supplemented with BA and NAA, kinetin and NAA, and BA, kinetin, and NAA at different concentrations. The number of shoots produced on the medium with 2 mg L~(-1) BA and 0,1 mg-L~(-1) NAA (5.0 shoots per explant in average) was higher than the other treatments. Rooting of in vitroshoots was carried out on MS basal medium containing indole-3-butyric acid (IBA) and NAA at different concentrations. The highest rate of rooting occurred when either shoots were first treated with 120 mgL~(-1) IBA solution for 24 h followed by transferring into the MS medium without plant growth regulators or cultured on MS medium supplemented with 4 mg-L~(-1) IBA and 2 mg L~(-1) NAA,, More roots were produced on MS medium containing both IBA and NAA than when they were used alone. The mixtures of peatand perlite at a ratio of 1:1 or 1:2 (v/v) were selected as the most suitable media for transplanting or adaptation of jojoba plantlets.