Monoclonal antibodies 410G and 33F against human and canine HER2 protein

摘要

A segment of the HER2 gene was amplified by PCR using a cDNA fragment (IMAGE human, Invitrogen, Carlsbad, CA) corresponding to the extracellular portion of the HER2 receptor (residues 76-309). The amplicon was cloned into the pQE30 expression vector. This plasmid was used to transform E. coli BL21(DE3) Star. The purification of recombinant protein was done using the A¨ KTAprime_ automated liquid chromatography system (GE Healthcare, Piscataway, NJ). The rHER2 protein in the final preparation was analyzed by 15% SDS-PAGE and quantified by the Bradford method.