Piezoresistive Microcantilever Biosensor Potentiometric Signal Transduction For Human Stress Measurement

摘要

This paper deals with the development of Piezoresistive Microcantilever biosensor and the signal transduction to detect human stress by using salivary alpha amylase activity. A Piezoresistive Microcantilever biosensor can be used to detect saliva-amylase activity by deflecting upon interaction with a specific receptor. By measuring the amount of bending the microcantilever beam experiences in response to interactions with the molecules, and the amount of analyte in the solution can be quantified. When the Microcantilever beam deflects it caused the stress change within the microcantilever beam and applied strain to the piezoresistor material thereby causing the resistance change which can be measured with the Wheatstone Bridge circuit. The Piezoresistive Microcantilever sensor integrated with transducer components converts the biochemical signal into measurable signal when it react with salivary amylase enzyme. The enzyme concentration signal is converted to a voltage signal by the transducer. The device was designed specifically that it enables the small resistivity change due to the enzymatic reaction to be measured.