High frequency of callusing from root explants of Assam Rice Collection

摘要

The regeneration of plants from cell and tissue culture is an important and essential component of biotechnology that is required for the genetic manipulation and improvement of plants. Rice is the world's single most important food crop and a primaryfood source for more than one third of world's population. Callusing and subsequent regeneration of rice from different explants were reported by Rueb et al. (1994), Seraj et al (1997), Wenjing et al. (1997) and Mukopadhyay et al (1997). However there are only a few reports on the induction of callus from root explants in rice. Mature seeds of Assam rice collection were obtained from Central Rice Research Institute Cuttack, along with japonica check Taipei 309, and were used for this study. For obtaining root explants, mature seeds were dehusked and sterilized under aseptic condition. MS medium was supplemented with 2 mgL~(-1)1AA or NAA (Indole acetic acid or Naphthalene acetic acid) and used for obtaining adventitious roots from rice seedlings. Eighteen to twenty days old roots from rice seedlings were selected as explants (Anju John and Prathapasenan, 1999). Effects of different carbon sources (sucrose/maltose) were also tested. Approximately 1.5 cm length root explants were transferred to callus induction medium (MS medium containing 2 mg 2, 4-DL~(-1)).