Identification of endogenous normalizers for serum MicroRNAs by microarray profiling: U6 small nuclear RNA is not a reliable normalizer

摘要

We read with great interest the results that the expression of circulating microRNA (miRNA) miR-122 was substantially higher in acetaminophen-induced acute liver injury (APAP-ALI) patients, compared to healthy controls, using quantitative realtime polymerase chain reaction (PCR) assays with U6 small nuclear RNA (snRNA) as an internal control, as reported by Starkey Lewis et al.1 However, serum miRNA expression profiles generated from a large number of human samples by our laboratory indicate that circulating U6 snRNA is not a reliable internal normalizer.