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A Method for GC-Olfactometry Panel Training

机译:GC-比色法小组培训的一种方法

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Odor-active compounds are commonly analyzed using gas chromatography-olfactometry (GC-O). However, there are only limited guidelines available for panelist training with this technique. In the current study, 29 volunteers were trained to detect, describe, and rate the intensity of odors. In addition, three GC-O methods, i.e., aroma extraction dilution method, detection frequency, and posterior intensity (PI), were used to evaluate the newly trained panelists' ability to analyze key compounds of kvass (fermented nonalcoholic drink) aroma. A five-step approach is proposed for training as follows: (1) introduction of the method; (2) vocabulary training using standard compounds and learning the use of the scale; (3) training with the reference mixture A; (4) training with the real product of interest-the beverage kvass; and (5) monitoring and further training of the panel. Following these steps, all panelists learned how to perform GC-O analysis. Some variances among subjects were observed; however, the background of thé trainees was found to be insignificant. Assessors for the "professional" GC-O panel were chosen for fiirther training and included people with a sensory and food science background, but also ordinary consumers. The PI method, where subjects rate odor intensity after a peak eluted, was found to provide a sufficient amount of data for key compound analysis. The method enabled easy data handling, provided valuable feedback for panel monitoring, and aided in the selection process to decide which assessors would be suitable for further training and placement on a professional panel.
机译:通常使用气相色谱-嗅觉法(GC-O)分析气味活性化合物。但是,只有有限的准则可用于使用此技术的小组成员培训。在当前的研究中,对29名志愿者进行了培训,以检测,描述和评估气味的强度。此外,还使用了三种GC-O方法,即香气提取稀释法,检测频率和后强度(PI)来评估新近训练的小组成员分析瓦斯酒(发酵非酒精饮料)香气中关键成分的能力。提出了以下五步训练方法:(1)方法介绍; (2)使用标准化合物进行词汇训练并学习量表的使用; (3)用参考混合物A进行训练; (4)使用感兴趣的真实产品-饮料格瓦斯进行培训; (5)监督和进一步培训专家组。按照这些步骤,所有小组成员都学习了如何执行GC-O分析。在受试者之间观察到一些差异;然而,这些学员的背景并不重要。选择了“专业” GC-O小组的评估员进行进一步培训,其中包括具有感官和食品科学背景的人员,以及普通消费者。发现PI方法可以让受试者对洗脱峰后的气味强度进行评分,从而为关键化合物分析提供了足够的数据量。该方法可以轻松处理数据,为专家组监控提供有价值的反馈,并有助于选择过程来确定哪些评估员适合进一步培训并放置在专业专家组上。

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