Separation of Dopamine and Epinephrine by a Novel Electrophoresis Technique with Nafion Membrane as Separation Column

摘要

A novel electrophoresis technique, in which a strip of perflurosulfonie-acid (Nafion 117) membrane was used to replace the conventional separation column and liquid buffer solution within, was developed and employed to separate the mixture of dopamineand epinephrine under a low separation voltage of 100 V with quadruple pulses amperornetry detection. It was showed that the so-called Nafion membrane electrophoresis could be one of very simple and easy method and has the potentiality to be used to separate and analyze some small organic biologic molecules.