Colchicine Inhibited the Expression of Tissue Inhibitor of Metalloprotenase-1 and lnterleukin-6 in Cultured Activated Hepatic Stellate Cells

摘要

Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of colchicine on HSCs growth were measured by MTT assay. Effects of colchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide microarray. Colchicine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colchicine, the expression of tissue inhibitor of metalloprotenase-1 (TIMP-1) and the expression of interleukin-6 (IL-6) in HSCswere down-regulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchicine's beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down-regulation of the expression of both TIMPf and IL-6 in HSCs.