Effects on CYP1A of the polycyclic musk tonalide (AHTN) in single and co-exposure with benzo(a)pyrene and 3,3'-4,4',5-pentachlorobiphenyl in the PLHC-1 fish cell line

摘要

The aim of this study was to investigate the interaction of a common polycyclic musk fragrance, namely tonalide (AHTN), with the detoxification gene family CYP1A, by looking at both gene transcription (cypla) and enzyme activity (7-ethoxyresorufin-O-deethylase; EROD) in the PLHC-1 fish cell line. Time-dependent (6 and 24 h) exposure experiments with three doses of AHTN (1 nM, 10 nM and 2μM) were performed. Co-exposure with known CYP1A inducers such as benzo[a]pyrene (B[a]P) and 3,3'-4,4',5-pentachlorobiphenyl (PCB 126) was also investigated. A slight induction of cypla gene transcription was observed after 6h, but not at 24 h. At 6h, 40-45% of reduction of cypla transcription was observed in co-exposure with B[a]P and PCB 126 compared with single inducers. Complete recovery was observed after 24 h. No effect on EROD activity by AHTN was observed in either single exposure and co-exposure. AHTN seemed not to affect CYP1A at the gene or enzyme level, but in co-exposure with inducers ATHN seems able to reduce detoxification capability within a short time.