Direct observation of the clockwise light-driven rotation of F_0F_1-ATP synthase complex

摘要

The purified thermophilic bacterium PS3 F_1beta_(10 X His-tag) is inserted into the F_0F_1-ATP synthases of chromatophores isolated from photosynthetic bacteria Rhodo-spirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protons-driving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by video-microscopic experiment, are connected via Maleimido-C_3-NTA to the reconstituted beta_(10 X His-tag) of F_0F_1-ATP synthases. The clockwise rotation of F_0F_1-ATP synthases driven by light is observed directly when viewed from the F_0 side to F_1. This system should be valuable for further studying the coupling property of F_0F_1-ATP synthase.