首页> 外文期刊>World Journal of Microbiology & Biotechnology >Antiviral activity of Cleome rosea extracts from field-grown plants and tissue culture-derived materials against acyclovir-resistant Herpes simplex viruses type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2)
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Antiviral activity of Cleome rosea extracts from field-grown plants and tissue culture-derived materials against acyclovir-resistant Herpes simplex viruses type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2)

机译:田间种植的植物和组织培养来源的玫瑰科油葵提取物对抗阿昔洛韦的单纯疱疹病毒1型(ACVr-HSV-1)和2型(ACVr-HSV-2)具有抗病毒活性。

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Extracts from Cleome rosea were investigated for their activity against acyclovir-resistant strains of Herpes simplex type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2). Methanolic and acidified (1% (v/v) HCl) methanolic extracts were prepared from field-grown plants and in vitro propagated plants, as well as from calli and cell suspension cultures. The extracts presented low cytotoxicity and caused virus titer reduction above 70%, with different mechanisms of action. Extracts from leaves of field-grown plants inhibited viral infection mainly by affecting the virus particle itself (virucidal effect), while extracts from calli acted mainly on cell receptors. On the other hand, all extracts evaluated affected the virus entry across the cell membrane and the intracellular viral replication at similar percentages, causing reduction on titers in the range of 68-90%. This study validated the potential use of in vitro materials as sources of antiherpetic agents from C. rosea.
机译:研究了玫瑰色油浸菌的提取物对单纯疱疹1型(ACVr-HSV-1)和2型(ACVr-HSV-2)抗阿昔洛韦的菌株的活性。甲醇和酸化(1%(v / v)HCl)甲醇提取物是从田间生长的植物和体外繁殖的植物以及愈伤组织和细胞悬浮培养物中制备的。提取物表现出低细胞毒性,并通过不同的作用机理使病毒滴度降低了70%以上。大田植物叶片的提取物主要通过影响病毒颗粒本身来抑制病毒感染(杀病毒作用),而愈伤组织的提取物主要作用于细胞受体。另一方面,所有评估的提取物都以相似的百分比影响病毒进入细胞膜和细胞内病毒复制,导致滴度降低68-90%。这项研究验证了体外材料作为玫瑰色念珠菌抗疱疹药来源的潜在用途。

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