Rapid in vitro Callogenesis and phytochemical screening of leaf and leaf callus of Ionidium suffruticosum, Ging.-a seasonal mulitpotent medicinal herb.

摘要

Protocol for rapid Callogenesis in Ionidium suffruticosum, Ging. has been standardized using leaf explant. Ionidium suffruticosum (Violaceae) is a seasonal, sporadic rare ethnobotanical herb used by traditional healers to treat several diseases. In order to formulate the micropropagation strategies of this plant, its various parts were used as explants for Callogenesis. Among the explants, the rapid callogenesis and proliferation was obtained from leaf explants on Murashige and Skoog (MS) medium supplemented with 2, 4-D (0.5 mg/L to 1.0 mg/L). The callus possessed a very high regenerative potential was referred as 'stock callus'. Different hormonal combinations often caused the transformation of the calli from soft crystalloid and translucent to compact, hard, yellowish and greenish forms. The phytochemical investigations showed that alkaloids, steroids and carbohydrates present in leaf (in vivo) and the same metabolites were also observed in leaf callus (in vitro).