pH effect of centre dot OH radical induced oxidation of phosphoryl-methionine in aqueous solution

摘要

STUDIES of radiation chemistry on methionine (Met) were very active in previous years because of its actions of radioprotection to biological systems by quenching centre dot OH radicals. On the other hand, people have paid more and more attention to the researches on the phosphorylation of the biological polymolecules. It is well known that phosphate ester is regarded as the depository of the biological energy due to its action of energy-reserving and energy-releasing in DNA and RNA. In recent years,it has been found that the reversible phosphorylation of proteins plays an important role not only in adjustment of enzyme activities and gene control, but also in occurence and treatment of tumors, radiation protection and anti-agent; and N-phos-phorylamino acids and its dipeptide derivatives with pharmaceutical interest have also been reported. Since amino acids are the elemental structural units of proteins, it is very important to study the radiolytical mechanism of phosphorylamino acids in order to clarify the actions of the phosphorylation of proteins.