Effects of expressing calmodulin antisense RNA on cell proliferation and transformation in human hepatoma cell lines

摘要

As the main intracellular receptor of Ca~(2+), calmodulin (CaM), which is extensively distributed in various types of eukaryotic cells, is ,a multifunctional protein regulating the cell growth, differentiation and transformation. Since the antisense RNA of CaM can more specifically inhibit CaM and has no pharmacological toxicity to cells compared with most of the antagonists of CaM, we constructed human hepatoma cell lines expressing CaM antisense RNA and analyzed their characteristics of growth, distribution of cell cycle, gene expression and the relationship with transformation under low levels of CaM in order to explore the effects of CaM on cell proliferation and transformation.