首页> 外文期刊>Virology >Mammalian reovirus L2 gene and lambda 2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing
【24h】

Mammalian reovirus L2 gene and lambda 2 core spike protein sequences and whole-genome comparisons of reoviruses type 1 Lang, type 2 Jones, and type 3 Dearing

机译:哺乳动物呼肠孤病毒L2基因和lambda 2核心刺突蛋白序列以及1型郎,2型琼斯和3型亲爱的呼肠孤病毒的全基因组比较

获取原文
获取原文并翻译 | 示例
       

摘要

The reovirus L2 genome segment encodes the core spike protein lambda2, which mediates enzymatic reactions in 5' capping of the viral plus-strand transcripts. Complete nucleotide-sequence determinations were made for the L2 genome segments of eight mammalian reoviruses, including the prototype isolates of serotypes 1 and 2: Lang (T1L) and Jones (T2J), respectively. Each L2 segment was found to be 3912 or 3915 bases in length. Partial nucleotide-sequence determinations were also made for the 3916-base L2 segment of reovirus type 3 Dearing (T3D), the prototype isolate of serotype 3. The whole-genome sequence of reovirus T3D was reported previously. The T1L L2 analysis represents completion of the whole-genome sequence of that isolate as well. The T2J L2 analysis leaves only the sequence of the M1 segment yet to be reported from the genome of that isolate. The T2J M1 sequence made available from analysis in another lab was used for initiating whole-genome comparisons of reoviruses T1L, T2J, and T3D in this report. The nine L2 gene sequences and deduced lambda2 protein sequences were used to gain further insights into the biological variability, structure, and functions of lambda2 through comparisons of the sequences and reference to the crystal structure of core-bound lambda2. Phylogenetic comparisons suggest the presence of three evolutionary lines of divergent L2 alleles among the nine isolates. Localized regions of conserved amino acids in the lambda2 crystal structure include active-site clefts of the RNA capping enzyme domains, sites of interactions between,2 domains within the pentameric spike structure, and sites of interaction between lambda2 subunits and other proteins in viral particles.
机译:呼肠孤病毒L2基因组片段编码核心刺突蛋白lambda2,该蛋白在病毒正链转录物的5'帽中介导酶促反应。对八种哺乳动物呼肠孤病毒的L2基因组片段进行了完整的核苷酸序列测定,分别包括血清型1和2的原型分离株:Lang(T1L)和Jones(T2J)。发现每个L2节段的长度为3912或3915个碱基。还对呼肠孤病毒3型原型分离株呼肠孤病毒3型亲爱的(T3D)的3916个碱基的L2节进行了部分核苷酸序列测定。呼肠孤病毒T3D的全基因组序列先前已有报道。 T1L L2分析也代表该分离株的全基因组序列的完成。 T2J L2分析仅留下尚未从该分离物的基因组报告的M1片段的序列。从另一实验室的分析中获得的T2J M1序列在本报告中用于呼肠孤病毒T1L,T2J和T3D的全基因组比较。通过比较序列并参考与核心结合的lambda2的晶体结构,使用了9个L2基因序列和推导的lambda2蛋白序列来进一步了解lambda2的生物学变异性,结构和功能。系统发育比较表明,在九个分离物中存在三个不同的L2等位基因进化线。 lambda2晶体结构中保守氨基酸的局部区域包括RNA加帽酶结构域的活性位点裂隙,五聚体刺突结构内2域之间的相互作用位点以及lambda2亚基与病毒颗粒中其他蛋白质之间的相互作用位点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号