Transfer RNA mimicry in a new group of positive-strand RNA plant viruses, the furoviruses: differential aminoacylation between the RNA components of one genome.

摘要

It is shown that the genomic RNAs of wheat soil-borne mosaic furovirus (SBWMV), beet soil-borne furovirus (BSBV), potato mop-top furovirus (PMTV), peanut clump furovirus (PCV) and Indian peanut clump furovirus (IPCV) all possess functional tRNA-likestructures (TLSs) that are capable of high-efficiency valylation. While the SBWMV, BSBV and PMTV TLSs are similar to those found in tymoviruses, the PCV and IPCV TLSs harbour an insertion of c 40 nucleotides between the 2 halves of the TLS. The valylated SBWMV and BSBV RNAs formed tight complexes with wheat germ EF-1alpha.GTP (Kd=2 to 11 nM), whereas valylated PMTV, PCV and IPCV RNAs bound EF-1alpha.GTP weakly (Kd >50 nm). The TLS of PCV RNA2 differs from PCV RNA1 in lacking the major valine identity nucleotide in the anticodon and consequently is capable of only very inefficient valylation. This is the first case of differential aminoacylation between the RNA components of one genome.