Immunophenotyping and quantitative evaluation of circulating lymphocytes of cattle of the Curraleiro breed

摘要

Moraes J. M., Brito L. A. B., Moura V. M. B. D., Ribeiro C. S., Guimaraes V. Y., Andrade D. F., Lobo J. R. & Fioravanti M. C. S. 2009. [Immunophenotyping and quantitative evaluation of circulating lymphocytes of cattle of the Curraleiro breed.] Imunofenotipagem e avaliacao quantitativa de linfocitos circulantes de bovinos da raca Curraleiro. Pesquisa Veterinaria Brasileira 29(4): 339-344. Escola de Veterinaria, Universidade Federal de Goias, Campus Samambaia, Cx. Postal 131, Goiania, GO 74001970, Brazil. E-mail: mmjulia_vet@yahoo.com.brThe aim of this study was to establish an immunological profile by marking and quantification of T and B lymphocytes in Curraleiro breed by immunocytochemistry. A total of 116 cattle was used, males and females of different ages, from two farms situated in the Goias State, Brazil. The animals were allotted into groups according to age, sex and origin. Blood samples were collected and processed in accordance with immunocytochemistry standard technique using lymphoid markers species-specific, as anti-CD3 (MM1A-BoCD3) and anti-LB (LCTB16A-clone B-B14), for T and B lymphocyte counting, respectively. The data were submitted to descriptive statistics, and to Kruskall Wallis and Mann-Whitney tests. The results showed decreased levels of leukocytes, lymphocytes, T lymphocytes and B lymphocytes along the age advance. Absolute values of leukocytes, lymphocytes and T lymphocytes were higher in males than females. None of the evaluated parameters were affected by differences of the management carried out at the two farms.