A simple method of preparing diverse neoagaro-oligosaccharides with beta-agarase

摘要

In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner,an enzymatic degradation method was developed,which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography.Agarose was degraded with AgaA at the optimized conditions,yielding 47% and 45% of neo-agarotetraose and neoagarohexaose,respectively.These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE.The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13C NMR spectroscopy.