Determination of structural peculiarities of dexran,pullulan and gamma-irradiated pullulan by fourier-transform IR spectroscopy

摘要

Deconvoluted IR-absorbance spectra of dextran,pullulan and gamma-irradiated pullulan were analyzed in order to find the most specific spectral peculiarities that allow oneto obtain information about the structure and conformation of these macromolecules in solvents that exhibit different influences on the system of intra-and inter molecular interactions.The changesin intensity and width of the IR bands at about 1040,1020 and,in the case of pullulan,also at 996 cm~(-1),were related to changes in conformation and short-range interactions of the polysaccharides.Furthermore,certainbands within the 1200-900 cm~(-1) region were considered as a characteristic for the type of glycosidic linkage.The results of the FTIR spectroscopy study allowed one to suggest a predominant cleavae of the alpha-(1->4) linkages upon the radiation-chemcial destruction of pullulan.