Expression of heat shock proteins HSP70 in pigs with chronic affliction oflungs and their detection by an immunocytochemical method

摘要

The presence of heat shock proteins (HSP70) in pig pulmonary tissue was detected using an immunocytochemical method. The pigs underwent a respiratory disease leading to coalescenses of visceral and parietal pleurae and fibrous reparation in lungs in the past. During the pre-slaughter handling, acute processes as congestion, edema, and cracking of alveolar sides occurred in animals with chronic affections of lungs. These pathological changes were accompanied by stress response in single alveolar cells, especially granulated pneumocytes (type II cells). An increased expression of HSP70 was found in pulmonary macrophages as well, their number in alveolar lumen markedly increased. HSP70 positive response in vessels was abnormally increased in fibrous coalescenses, while other fibrous tissue was quite negative.