Sister-chromatid exchanges induced by metal containing emission in sheepperipheral lymphocytes after in vivo and in vitro exposure

摘要

The effect of ill vivo feeding and by standard in vitro assay of metal-containing emission originated from aluminum proceeding factory were tested to induce sister-chromatid exchanges (SCE) in sheep peripheral lymphocytes. Content of the emission tested, was performed by A1, Cd, As, Mn, Pb, Cu, Zn, Fe, Na, Ca, and Mg; majority of them as sulphide, sulphate, oxide or fluoride form. The experimental animals were given a daily dose of emission of either 0.75 g or 1.5 g per animal, for 1 year. The experiments were aimed to induce the chronic fluorosis. The occurrence of SCEs in the experimental group was higher (reaching the statistical significance at a dose 1.5 g/animal). The emission was found to reduce the proliferation index as well as to influence the mitotic index. The experiment was compared with sheep peripheral lymphocytes in the in vitro assay. The emission was dissolved and neutralised according to the standard method and analysed spectrophotometrically. Level of SCE was investigated at three concentrations of 30, 60 and 90 mug/ml, respectively with and without of S 9 mix metabolic activation. The lowest concentration used corresponds to daily oral intake for sheep grazing on contamined area. Results from both of in vitro assays were similar; the significant increasing of SCE started at the concentration of 60 mug/ml (p < 0.01) but more perspicuous results were seen without of S 9. No significant decrease was found in the induction of proliferation index. Indirect verification for genotoxic influence of the emission tested has been given by local veterinarians keeping evidence of developmental abnormalities. Total percentage of developmental defects in the district represented 9.58% all of the developmental disorders in cattle reported in the middle Slovakia territory at the given time.