Expression, purification and characterization of endo-type chitosanase of Aspergillus sp CJ22-326 from Escherichia coli

Li SL; Chen L; Wang C; Xia WS

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Expression, purification and characterization of endo-type chitosanase of Aspergillus sp CJ22-326 from Escherichia coli

机译：大肠杆菌曲霉CJ22-326内切型壳聚糖酶的表达，纯化和鉴定

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An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases. (C) 2008 Elsevier Ltd. All rights reserved.

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《Carbohydrate research》 |2008年第17期|共4页
作者
Li SL; Chen L; Wang C; Xia WS;
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正文语种 eng
中图分类 Q629;
关键词
Aspergillus; endo-Type chitosanase; High chitosanolytic activity; Gene cloning; His-Tag purification;

机译：曲霉;内切型壳聚糖酶;高壳聚糖分解活性;基因克隆;His-Tag纯化;

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专利

1. Expression, purification and characterization of endo-type chitosanase of Aspergillus sp CJ22-326 from Escherichia coli [J] . Li SL, Chen L, Wang C, Carbohydrate research . 2008,第17期

机译：大肠杆菌曲霉CJ22-326内切型壳聚糖酶的表达，纯化和鉴定
2. Purification and characterization of two types of chitosanase from Aspergillus sp. CJ22-326. [J] . Chen Xiao e, Xia WenShui, Yu XiaoBin Food research international . 2005,第3期

机译：两种曲霉菌壳聚糖酶的纯化和鉴定。 CJ22-326。
3. Improved extracellular expression and high-cell-density fed-batch fermentation of chitosanase from Aspergillus Fumigatus in Escherichia coli [J] . Huang Liang, Wang Qinhong, Jiang Sijing, Bioprocess and Biosystems Engineering . 2016,第11期

机译：改进了烟曲霉中壳聚糖酶在大肠杆菌中的细胞外表达和高细胞密度分批补料发酵
4. Cloning, expression, purification, and characterization of an organophosphate-degrading enzyme in Escherichia coli [C] . Jinbin Wang, Dachao Chen, Dongxiao Hu, International Conference on Energy, Environment and Sustainable Development . 2013

机译：在大肠杆菌中有机磷酸盐降解酶的克隆，表达，纯化和表征
5. Cloning, expression, purification, and characterization of a glycoside hydrolase family 44 cellulase from Clostridium acetobutylicum in Escherichia coli. [D] . Shilling, Taran Christopher. 2007

机译：从大肠杆菌中的丙酮丁醇梭菌糖苷水解酶家族44纤维素酶的克隆，表达，纯化和表征。
6. Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp. in Escherichia coli [O] . Yuying Sun, Jiquan Zhang, Shujun Wang 2015

机译：微生物壳聚糖酶的异源表达和有效分泌。在大肠杆菌中
7. A novel chitosanase from Bacillus sp. Strain K17: Gene cloning and expression in Escherichia coli [O] . R. Yatsunami, Y. Sakihama, M. Suzuki, 2002

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8. Purification and Characterization of Acinetobacter Calcoaceticus 4-Hydroxybenzoate 3-Hydroxylase after its Overexpression in Escherichia coli [R] . Fernandez, J., DiMarco, A. A., Ornston, L. N., 1995

机译：醋酸钙不动杆菌4-羟基苯甲酸3-羟化酶在大肠杆菌中过表达后的纯化和鉴定

Expression, purification and characterization of endo-type chitosanase of Aspergillus sp CJ22-326 from Escherichia coli

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