Structure of a new ribitol teichoic acid-like O-polysaccharide of a serologically separate Proteus vulgaris strain, TG 276-1 classified into a new Proteus serogroup O53

摘要

An unusual ribitol teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified Proteus viularis strain TG 276-1. Structural studies using chemical analyses and 2D H-1 and(13)C NMR spectroscopy showed that the polysaccharide is a zwitterionic polymer with a repeating unit containing 2-acetamido-4amino-2,4,6-trideOXy- D -galactose (D-FucNAc4N) and two iD-ribitol phosphate (D-Rib-ol-5-P) residues and having the following structure: -> 3)-beta-D-FucpNAc4N-(1 -> 1)-D-Rib-ol-5-P-(O -> 1)-D-Rib-ol-5-P-(O ->, where the non-glycosylated ribitol residue is randomly mono-O-acetylated. Based on the unique 0-polysaccharide structure and the finding that the strain studied is serologically separate among Proteus bacteria, we propose to classify P. vulgaris strain TG 276-1 into a new Proteus serogroup, O53. (c) 2007 Elsevier Ltd. All rights reserved.