STRUCTURAL STUDIES OF THE ACIDIC EXOPOLYSACCHARIDE PRODUCED BY A MUCOID STRAIN OF BURKHOLDERIA CEPACIA, ISOLATED FROM CYSTIC FIBROSIS

摘要

The acidic exopolysaccharide produced by a mucoid strain of Burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. Based on the sugar composition and permethylation analyses, supported by GLC-MS and NMR spectroscopy analyses, the repeating-unit of the polysaccharide was established as --> 3)-beta-D-Glc p-(1 --> 3)-[4,6-O-(1-carboxyethylidene)]-alpha-D-Gal p-(1 -->. (C) 1996 Elsevier Science Ltd. [References: 26]