Addition of glycerol for improved methylation linkage analysis of polysaccharides

摘要

A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides.Neutral,acidic,high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC-MS.All four types of polysaccharides showed significant increases in derivatization following presolubilization as measured by recovery of partially methylated alditol acetates.