Somatic embryogenesis in groundnut: a comparison of sixty-nine Indian genotypes

摘要

Immature zygotic embryos were cultured on modified MS medium with 1 mg/litre of NAA and 15, 20 or 25 mg/litre of 2,4-D. Somatic embryogenesis was observed in all the genotypes studied. It and the number of somatic embryos per explant were significantly influenced by the difference in genotypes, level of 2,4-D and the interaction between the two. The number of somatic embryos per explant varied from 1 to 15.8 and the frequency of shoot regeneration from 45 to 100%. Significant differences were observed between the genotypes in the percentage of shoot induction from somatic embryos and the number of shoots per embryo. The mean number of shoots per somatic embryo varied between 1 and 5.9. Of 17 genotypes studied for rooting, 14 showed 100%. The number of roots per shoot varied between 1 and 15. It appears that strong genotypic regulation exists for the somatic embryogenesis and shoot regeneration in groundnut. The high overall response confirms the suitability of the protocol over a wide range of genotypes.