Structure and gene cluster of the O-antigen of Salmonella enterica O60 containing 3-formamido-3,6-dideoxy-d-galactose

摘要

An O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Salmonella enterica O60 strain G1462, and the following unique structure of the O-unit was determined by chemical analyses along with 2D ~1H and ~(13)C NMR spectroscopy:{A figure is presented}. where Fuc3NFo stands for 3-formamido-3,6-dideoxygalactose. The structure established is in agreement with the O-antigen gene cluster of S. enterica O60, which contains putative genes for the synthesis of GDP-d-Man and dTDP-d-Fuc3NFo, three glycosyltransferase genes, and two O-unit-processing genes (wzx and wzy).