Analysis of chemical composition, structure of Grifolafrondosa polysaccharides and its effect on skin TNF-α levels, lgG content, T lymphocytes rate and caspase-3 mRNA

摘要

The polysaccharide was extracted from Grifola frondosa mushroom. The chemical composition of G. frondosa polysaccharide was analyzed by the gas chromatography-mass spectroscopy (GC-MS). The G. frondosa polysaccharide was found to consist of glucose, mannose and galactose with a molar ratio of 6.5:1:2.6. The structure of G. frondosa polysaccharide was characterized by infrared (1R) spectrum, 1D NMR and 2D NMR spectroscopy. The absorption peaks of 1415 cm~(-1),1154cm~(-1) and 851 cm~(-1) suggested the polysaccharide consisted of pyranoside. It was found that G. frondosa polysaccharide was a heteropolysac-charide consisting of the repeating disaccharide unit. Comparing control rats, G. frondosa polysaccharide treatment markedly enhanced skin TNF-a levels, reduced lgG content, T lymphocytes rate and caspase-3 mRNA expression in group 2 rats. Administration of G. frondosa polysaccharide significantly reduced skin levels of TNF-a, increased lgG content, T lymphocytes rate and caspase-3 mRNA expression in groups 3 and 4 rats. In conclusion, G. frondosa polysaccharides are beneficial for those with skin diseases.