Enzymatic production of N-acetyl chitooligosaccharides by crude enzyme derived from Paenibacillus illioisensis KJA-424

摘要

Production of N-acetyl-D-glucosamine (GlcNAc) and N-acetyl chitooligosaccharides is important to the food,agriculture and biotechnology sectors,where they can be employed as both plant growth promoters and agents of biocontrol for plant pathogens.N-Ac-etyl-D-glucosamine (GlcNAc) and N-acetyl chitooligosaccharides were produced from colloidal chitin by use of crude enzyme obtained from Paenibacillus illinoisensis KJA-424.The production rate of GlcNAc increased continuously during incubation,while that of GlcNAc oligomers declined.The maximum production of GlcNAc was 1.71 mg mL~(-1) (yield of 62.2%) after 24 h of incubation.At the same time chitobiose [(GlcNAc)_2],chitotriose [(GlcNAc)_3],chitoheptose [(GlcNAc)_7] and chitooctose [(GlcNAc)_8] were 0.13 (yield of 4.9%),0.03 (yield of 1.2%),0.01 (yield of 4.1%) and 0.24 mg mL~(-1) (yield of 9.6%),respectively.The rate of total N-acetyl chitooligosaccharide production decreased by over 44% and 87% after 2 and 24 h of incubation,respectively.The multi-chitinolytic enzyme complex produced by Paenibacillus illinoisensis KJA-424 is effective in the production of GlcNAc and N-acetyl chitooligosaccharides,facilitating its potential use in industrial applications.